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2. | | CARNEIRO NETO, T. F. de S.; REBOUÇAS, P. L. O.; XAVIER, C. F.; COELHO, W. S. S.; KIILL, L. H. P. Espectro polínico de Centris aenea (Lepeletier, 1841) (Apidae: Centridini) em cultivo de aceroleira. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 13., 2018, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2018. p. 53-58. (Embrapa Semiárido. Documentos, 283). Biblioteca(s): Embrapa Semiárido. |
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3. | | CARNEIRO NETO, T. F. de S.; XAVIER, C. F.; SANTOS, M. V. L.; SILVA, R. C. S. da; KIILL, L. H. P. Viabilidade polínica e receptividade estigmática de duas variedades de aceroleira. In: JORNADA DE INICIAÇÃO CIENTÍFICA DA EMBRAPA SEMIÁRIDO, 12., 2017, Petrolina. Anais... Petrolina: Embrapa Semiárido, 2017. p. 27-32. (Embrapa Semiárido. Documentos, 279). Biblioteca(s): Embrapa Semiárido. |
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4. | | PEREIRA, D. S.; OLIVEIRA, M. E. C.; MOURA, M. J.; CARVALHO, W. de A. de; ARRUDA, H. M.; COSTA, L.; DEUS, J. C. S. de; MEDEIROS, A. C. de; SILVA, R. A. da; MARACAJÁ, P. B.; HOLANDA-NETO, J. P. de; SAID, P.; XAVIER, C. F.; CARVALHO, C. J. R. de; FONSECA, V. L. I.; SOUZA JÚNIOR, P. A. de. Monitoramento de abelhas africanizadas (Apis mellifera L.) usando tecnologia de rádio frequência (RFID) em Belém, Pará. Acta Apicola Brasilica, v. 10, e9654, 2022. Biblioteca(s): Embrapa Agroindústria Tropical; Embrapa Amazônia Oriental. |
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Registro Completo
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
05/02/2024 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 4 |
Autoria: |
DUARTE, M. F.; ANDRADE, I. A. de; SILVA, J. M. F.; MELO, F. L. de; MACHADO, A. M.; INOUE-NAGATA, A. K.; NAGATA, T. |
Afiliação: |
MACÁRIA FERREIRA DUARTE, UNIVERSIDADE DE BRASÍLIA; IKARO ALVES DE ANDRADE, UNIVERSIDADE DE BRASÍLIA; JOÃO MARCOS FAGUNDES SILVA, UNIVERSIDADE DE BRASÍLIA; FERNANDO LUCAS DE MELO, UNIVERSIDADE DE BRASILIA; ANA MARIA MACHADO, COMPANHIA DE SANEAMENTO AMBIENTAL DO DF; ALICE KAZUKO INOUE NAGATA, CNPH; TATSUYA NAGATA, UNIVERSIDADE DE BRASÍLIA. |
Título: |
Metagenomic analyses of plant virus sequences in sewage water for plant viruses monitoring. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Tropical Plant Pathology, v. 48, p. 408-416, 2023. |
DOI: |
10.1007/s40858-023-00575-8 |
Idioma: |
Inglês |
Conteúdo: |
Frequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identifcation of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, highthroughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphafexiviridae, Betafexiviridae, losteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources. The potential use of sewage for a virus survey is discussed in this study. MenosFrequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identifcation of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, highthroughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphafexiviridae, Betafexiviridae, losteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources.... Mostrar Tudo |
Palavras-Chave: |
High-throughput sequencing. |
Thesagro: |
Vírus. |
Thesaurus NAL: |
Wastewater. |
Categoria do assunto: |
S Ciências Biológicas |
Marc: |
LEADER 02294naa a2200241 a 4500 001 2161643 005 2024-02-05 008 2023 bl uuuu u00u1 u #d 024 7 $a10.1007/s40858-023-00575-8$2DOI 100 1 $aDUARTE, M. F. 245 $aMetagenomic analyses of plant virus sequences in sewage water for plant viruses monitoring.$h[electronic resource] 260 $c2023 520 $aFrequent monitoring of emerging viruses of agricultural crops is one of the most important missions for plant virologists. A fast and precise identifcation of potential harmful viruses may prevent the occurrence of serious epidemics. Nowadays, highthroughput sequencing (HTS) technologies became an accessible and powerful tool for this purpose. The major discussion of this strategy resides in the process of sample collection, which is usually laborious, costly and nonrepresentative. In this study, we assessed the use of sewage water samples for monitoring the widespread, numerous, and stable plant viruses using HTS analysis and RT-qPCR. Plant viruses belonged to 12 virus families were found, from which Virgaviridae, Solemoviridae, Tymoviridae, Alphafexiviridae, Betafexiviridae, losteroviridae and Secoviridae were the most abundant ones with more than 20 species. Additionally, we detected one quarantine virus in Brazil and a new tobamovirus species. To assess the importance of the processed foods as virus release origins to sewage, we selected two viruses, the tobamovirus pepper mild mottle virus (PMMoV) and the carlavirus garlic common latent virus (GarCLV), to detect in processed food materials by RT-qPCR. PMMoV was detected in large amount in pepper-based processed foods and in sewage samples, while GarCLV was less frequent in dried and fresh garlic samples, and in the sewage samples. This suggested a high correlation of virus abundance in sewage and processed food sources. The potential use of sewage for a virus survey is discussed in this study. 650 $aWastewater 650 $aVírus 653 $aHigh-throughput sequencing 700 1 $aANDRADE, I. A. de 700 1 $aSILVA, J. M. F. 700 1 $aMELO, F. L. de 700 1 $aMACHADO, A. M. 700 1 $aINOUE-NAGATA, A. K. 700 1 $aNAGATA, T. 773 $tTropical Plant Pathology$gv. 48, p. 408-416, 2023.
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